Pluripotent stem cells provide access to a large variety of human cell types. However, stem cell culture is often heterogeneous and efficient differentiation and purification of stem cell-derived cells are limited to relatively few examples. Via genetic engineering, we have generated stem cell reporter lines that enable the detection and purification of retinal ganglion cells (RGCs), an essential cell type for vision. Through modulation of known signaling pathways, we report an improved RGC differentiation protocol for high yields of purified RGCs. We also describe an siRNA protocol for exploration of signaling pathways in human RGCs.
An Induced Pluripotent Stem Cell Patient Specific Model of Complement Factor H (Y402H) Polymorphism Displays Characteristic Features of Age‐Related Macular Degeneration and Indicates a Beneficial Role for UV Light Exposure
Age-related macular degeneration (AMD) is the one of the most common forms of blindness. Drugs that treat wet AMD have been a major breakthrough; however, there is currently no treatment for the dry form. One of the problems with studying AMD is that the affected retinal tissue is difficult to obtain, there are no animal models that faithfully mimic the disease and human trials are long and costly. Herein, we report creation of a disease model for AMD patients with the most common genetic risk factor for the disease. We provide strong evidence to show that this model mimics the key features of AMD and can be used to test new therapies and to better understand the pathology of disease and the role of environmental, dietary, and lifestyle factors.
The overexpression of basic fibroblast growth factor boosts bone fracture healing capacity of mesenchymal stem cells via the secretion of paracrine-acting factors