Research Tools

Interesting Stem Cells Sites

Stem Cell Resources.org - These Science of Education

A division of the Bioscience Network (BSN), a publisher of online science education materials, aiming to provide timely, reliable, high-quality and scientifically credible stem cell information for the educational community worldwide.

Stem Cell Banks and Registries

Adult Mesenchymal Stem Cell Resource
The Tulane Center for Gene Therapy has received a grant funded by the NIH to establish a center for preparation, quality testing, and distribution of Bone Marrow Stromal Cells, also known as Mesenchymal Stem Cells, to academic researchers worldwide upon request.  The resource provides well-characterized human adult stem cells, rat stem cells, and mouse stem cells, some of which are also available containing fluorescent proteins such as GFP (cytoplasmic expression), MitoRed or MitoBlue (mitochondrial expression), and some MSCs with a combination of GFP and either MitoRed or MitoBlue.

ATCC Stem Cell Center
Includes Human Embryonic Stem Cells, mouse germ line competent embryonic stem cells, mouse modified In Vitro embryonic stem cells, human and mouse Feeder Layer cells, and embryomal carcinoma cell lines.  Characterizes and distributes stem cell lines, and develops and optimizes research methodologies and reagents.

BLCB The Stem Cell Bank of Barcelona at the Center of Regenerative Medicine in Barcelona (CMR[B])
Dedicated to the derivation, maintenance, characterization and preservation of human embryonic stem cells with the aim of advancing research activities in the area of regenerative medicine. The main objective of the BLCB is the derivation of xenobiotics-free hESC lines under GMP-compliant conditions that are applicable in clinical research

ECACC European Collection of Cell Cultures
A general Cell Culture collection to service the research community.  Currently holds over 40,000 cell lines representing 45 different species; among these are some mouse and human stem and progenitor cells.

hESCreg  European Human Embryonic Stem Cell Registry
Contain information on the characteristics of the stem cell lines, where they came from and relevant contact data, as well as news of interesting developments such as clinical trials and information on human embryonic stem cell lines obtained from EU-funded projects.

ISCI International Stem Cell Initiative Stem Cell Registry
A project of the International Stem Cell Forum, to systematically study human embryonic stem cells in an effort to establish an international set of standards for characterization.

ISCR International Stem Cell Registry

The mission of the University of Massachusetts International Stem Cell Registry is to provide a searchable, comprehensive database that includes published and validated unpublished information on all human ES cell lines as well as other pluripotent stem cell lines. 

NHNSCR National Human Neural Stem Cell Resource
Provides neural stem cells harvested from the post-natal, post-mortem, human brain to the research community for stem cell research. Several brain areas as well as cultures from normal and genetically mutant specimens are represented in the Resource.

NIH National Institutes of Health Embryonic Stem Cell Registry
Lists the derivations of stem cells that are eligible for U.S. federal funding.  Provide investigators with a unique NIH Code for each cell line that must be used when applying for NIH funding and contact information to facilitate investigators' acquisition of stem cells.

NSCB National Stem Cell Bank
Established at WiCell in 2005 to acquire, characterize and distribute the human embryonic stem cell lines and their sub-clones available for use in U.S. federally funded research programs and to provide technical support to the hES cell research community.

Riken BioResource Center Cell Bank
A general cell line resource in Japan, which includes a  variety of mouse germ line and embryonic stem cell lines, including modified lines and lines established by nuclear transfer technique.  .

The Stem Cell Community – Human Embryonic Stem Cell Lines
A searchable database of more than 250 human embryonic stem cell lines worldwide.

UKSCB United Kingdom Stem Cell Bank
Provides a repository for human stem cell lines of all types, and has been developed to supply well characterized cell lines under appropriate and accredited quality systems both for basic research and for the development of clinical applications.

Policy & Ethics Guidelines

The following are current Stem Cell policies and ethical guidelines:

 

The American Association for the Advancement of Science
The AAAS site includes numerous resources related to stem cell scientific, ethical, and policy issues, including the findings and recommendations of the AAAS/ICS Report on Stem Cell Research.

The National Academies
The National Academies’ Guidelines for Human Embryonic Stem Cell Research can be found at this site, along with several other free publications related to stem cell science and policy.

The National Institute of Health
The NIH resource for stem cell research website provides information and numerous documents related to the federal policies associated with human embryonic stem cell research.

The California Institute for Regenerative Medicine
The CIRM website contains information about the California Stem Cell Research and Cures Act (California/Prop 71).

The Science Network’s Stem Cell Symposium
The Science Network has archived webcasts from their symposium “Stem Cells: Science Ethics and Politics and the Crossroads”

Online Protocols

Below are links to Embryonic Stem Cell and Adult Stem Cell online protocols:

Embryonic Stem Cells

National Stem Cell Bank (NSCB)
Standard Operating Procedures (SOPs) used for the production and testing of cell lines distributed by the NSCB.

WiCell Research Institute
Protocols followed in the WiCell production and distribution laboratory to successfully grow and expand hESC lines.

Cold Spring Harbor Protocols
New and classic research techniques for ESC research, with a searchable database.

Current Protocols in Stem Cell Biology
Online edition of the laboratory manual from Wiley InterScience, updated monthly.  Subscription required.

Thomson Lab Protocols
hESC, MEF, and iPS protocols from James Thomson’s lab at the University of Wisconsin.

University of California, San Francisco
Protocols used by the Human Embryonic Stem Cell Biology Program to maintain their stem cell lines.

The Murine Embryonic Stem Cell Core, The Siteman Cancer Center
mESC protocols in use at the Washington University School of Medicine core facility.

Protocol Online
Research protocols in a variety of life science fields, contributed by researchers worldwide and collected from the web, with discussion forums.

Molecular Station
A compilation of ESC protocols from internet sources.

Invitrogen
Protocols for isolation, characterization, Differentiation, and expansion of ESCs  from Invitrogen.

BresaGen, Inc. 2004 Methods Manual
Methods for culturing BresaGen’s hESC lines.

Geron Corporation Protocols
Protocols for the maintenance of hESCs in Feeder Free Conditions

Adult Stem Cells

Cold Spring Harbor Protocols
New and classic research techniques for stem cell research, with a searchable database.

Current Protocols in Stem Cell Biology
Online edition of the laboratory manual, updated monthly.  Subscription required.

Protocol Online
Research protocols in a variety of life science fields, contributed by researchers worldwide and collected from the web, with discussion forums.

The National Human Neural Stem Cell Resource
Stem cell harvest protocols used at the National Human Neural Stem Cell Resource, providing neural stem cells harvested from the post-natal, post-mortem, human brain to the research community.  

Invitrogen
Protocols for isolation, characterization, differentiation, and expansion of adult stem cells from Invitrogen.

R&D Systems Protocols
Methods and products from R&D Systems.


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