Online Protocols

Below are links to Embryonic Stem Cell and Adult Stem Cell online protocols:

Embryonic Stem Cells

National Stem Cell Bank (NSCB)
Standard Operating Procedures (SOPs) used for the production and testing of cell lines distributed by the NSCB.

WiCell Research Institute
Protocols followed in the WiCell production and distribution laboratory to successfully grow and expand hESC lines.

Cold Spring Harbor Protocols
New and classic research techniques for ESC research, with a searchable database.

Current Protocols in Stem Cell Biology
Online edition of the laboratory manual from Wiley InterScience, updated monthly.  Subscription required.

Thomson Lab Protocols
hESC, MEF, and iPS protocols from James Thomson’s lab at the University of Wisconsin.

University of California, San Francisco
Protocols used by the Human Embryonic Stem Cell Biology Program to maintain their stem cell lines.

The Murine Embryonic Stem Cell Core, The Siteman Cancer Center
mESC protocols in use at the Washington University School of Medicine core facility.

Protocol Online
Research protocols in a variety of life science fields, contributed by researchers worldwide and collected from the web, with discussion forums.

Molecular Station
A compilation of ESC protocols from internet sources.

Invitrogen
Protocols for isolation, characterization, Differentiation, and expansion of ESCs  from Invitrogen.

BresaGen, Inc. 2004 Methods Manual
Methods for culturing BresaGen’s hESC lines.

Geron Corporation Protocols
Protocols for the maintenance of hESCs in Feeder Free Conditions

Adult Stem Cells

Cold Spring Harbor Protocols
New and classic research techniques for stem cell research, with a searchable database.

Current Protocols in Stem Cell Biology
Online edition of the laboratory manual, updated monthly.  Subscription required.

Protocol Online
Research protocols in a variety of life science fields, contributed by researchers worldwide and collected from the web, with discussion forums.

The National Human Neural Stem Cell Resource
Stem cell harvest protocols used at the National Human Neural Stem Cell Resource, providing neural stem cells harvested from the post-natal, post-mortem, human brain to the research community.  

Invitrogen
Protocols for isolation, characterization, differentiation, and expansion of adult stem cells from Invitrogen.

R&D Systems Protocols
Methods and products from R&D Systems.


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