Peripheral nerve injury affects millions of patients each year resulting in reduced quality of life, and billions of dollars in health care costs and lost work capacity. Surgical intervention with autografts work reasonably well for small lesions but for larger injuries, the functional outcomes are often unfavorable. Combining a surgical approach with contemporary developments in tissue engineering offers a potentially rewarding way forward. This study indicates that olfactory derived stem cells can have a regenerative effect on peripheral nerve repair in vivo when delivered on a novel biomaterial consisting of a collagen and laminin functionalized hyaluronic acid nerve guidance conduit.
Human pluripotent stem cells (hPSCs) have recently been differentiated into complex kidney organoids. Generation of podocytes—the filtering cells of the kidney—in these organoids is of great interest, because podocytes are highly specialized cells that regenerate poorly in vivo and are challenging to culture using conventional methods. However, hPSC-podocytes differ from cultured podocytes in appearance and gene expression, and it is important to determine the developmental stage of hPSC-podocytes and their ability to recapitulate disease phenotypes. In this study, we address these questions by comparing hPSC-podocytes to developing podocytes in human and mouse kidneys. We demonstrate that hPSC-podocytes mimic developing podocytes in vivo at the capillary loop stage of glomerular maturation, both structurally and in disease phenotype, providing new insight into the genetic mechanisms of podocyte specialization. This establishes a powerful new tool for genetic studies of human podocyte development and disease.
The overexpression of basic fibroblast growth factor boosts bone fracture healing capacity of mesenchymal stem cells via the secretion of paracrine-acting factors