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CTS Cell Transplant Society

An international non for profit, incorporated collegial association of scientists with background and/or interest in the field of cellular transplantation.  The CTS  exists to promote research and collaboration in cellular transplantation.

ASNTR American Society for Neural Therapy and Repair

Composed of basic and clinical neuroscientists who utilize a variety of technologies to better understand the way the nervous system functions and establish new procedures for its repair in response to trauma or neurodegenerative disease.

10 Most Frequently Read Articles from Stem Cells Journal - Aug 2009

The 20 Most-Frequently Read Articles
in Stem Cells during September 2008 thru August 2009 -- updated monthly

Most-read rankings are recalculated at the beginning of the month and are based on full-text and pdf views.

 

1. Giselle Chamberlain, James Fox, Brian Ashton, Jim Middleton
  Concise Review: Mesenchymal Stem Cells: Their Phenotype, Differentiation Capacity, Immunological Features, and Potential for Homing
  Stem Cells Nov 01, 2007; 25: 2739-2749.
(In "TISSUE-SPECIFIC STEM CELLS")   [Abstract] [Full Text] [PDF]
  (Read 6151 times)
2. Donald G. Phinney, Darwin J. Prockop
  Concise Review: Mesenchymal Stem/Multipotent Stromal Cells: The State of Transdifferentiation and Modes of Tissue Repair—Current Views
  Stem Cells Nov 01, 2007; 25: 2896-2902.
(In "TRANSLATIONAL AND CLINICAL RESEARCH: MESENCHYMAL STEM CELLS SERIES")   [Abstract] [Full Text] [PDF]
  (Read 5584 times)
3. Prashant Mali, Zhaohui Ye, Holly H. Hommond, Xiaobing Yu, Jeff Lin, Guibin Chen, Jizhong Zou, Linzhao Cheng
  Improved Efficiency and Pace of Generating Induced Pluripotent Stem Cells from Human Adult and Fetal Fibroblasts
  Stem Cells Aug 01, 2008; 26: 1998-2005.
(In "EMBRYONIC STEM CELLS/INDUCED PLURIPOTENT STEM CELLS")   [Abstract] [Full Text] [PDF]
  (Read 5218 times)
4. Lindolfo da Silva Meirelles, Arnold I. Caplan, Nance Beyer Nardi
  In Search of the In Vivo Identity of Mesenchymal Stem Cells
  Stem Cells Sep 01, 2008; 26: 2287-2299.
(In "The Stem Cell Niche")   [Abstract] [Full Text] [PDF]
  (Read 5175 times)
5. Peter J. Psaltis, Andrew C.W. Zannettino, Stephen G. Worthley, Stan Gronthos
  Concise Review: Mesenchymal Stromal Cells: Potential for Cardiovascular Repair
  Stem Cells Sep 01, 2008; 26: 2201-2210.
(In "TRANSLATIONAL AND CLINICAL RESEARCH")   [Abstract] [Full Text] [PDF]
  (Read 4840 times)
6. Stephanie M. Willerth, Tracy E. Faxel, David I. Gottlieb, Shelly E. Sakiyama-Elbert
  The Effects of Soluble Growth Factors on Embryonic Stem Cell Differentiation Inside of Fibrin Scaffolds
  Stem Cells Sep 01, 2007; 25: 2235-2244.
(In "EMBRYONIC STEM CELLS")   [Abstract] [Full Text] [PDF]
  (Read 4477 times)
7. Vinagolu K. Rajasekhar, Martin Begemann
  Concise Review: Roles of Polycomb Group Proteins in Development and Disease: A Stem Cell Perspective
  Stem Cells Oct 01, 2007; 25: 2498-2510.
(In "STEM CELL GENETICS AND GENOMICS")   [Abstract] [Full Text] [PDF]
  (Read 4466 times)
8. Andreas Schäffler, Christa Büchler
  Concise Review: Adipose Tissue-Derived Stromal Cells—Basic and Clinical Implications for Novel Cell-Based Therapies
  Stem Cells Apr 01, 2007; 25: 818-827.
(In "TISSUE-SPECIFIC STEM CELLS")   [Abstract] [Full Text] [PDF]
  (Read 4157 times)
9. Yaojiong Wu, Liwen Chen, Paul G. Scott, Edward E. Tredget
  Mesenchymal Stem Cells Enhance Wound Healing Through Differentiation and Angiogenesis
  Stem Cells Oct 01, 2007; 25: 2648-2659.
(In "TRANSLATIONAL AND CLINICAL RESEARCH")   [Abstract] [Full Text] [PDF]
  (Read 4045 times)
10. Ornella Parolini, Francesco Alviano, Gian Paolo Bagnara, Grozdana Bilic, Hans-Jörg Bühring, Marco Evangelista, Simone Hennerbichler, Bing Liu, Marta Magatti, Ning Mao, Toshio Miki, Fabio Marongiu, Hideaki Nakajima, Toshio Nikaido, C. Bettina Portmann-Lanz, Venkatachalam Sankar, Maddalena Soncini, Guido Stadler, Daniel Surbek, Tsuneo A. Takahashi, Heinz Redl, Norio Sakuragawa, Susanne Wolbank, Steffen Zeisberger, Andreas Zisch, Stephen C. Strom
  Concise Review: Isolation and Characterization of Cells from Human Term Placenta: Outcome of the First International Workshop on Placenta Derived Stem Cells
  Stem Cells Feb 01, 2008; 26: 300-311.
(In "TISSUE-SPECIFIC STEM CELLS")   [Abstract] [Full Text] [PDF]
  (Read 3954 times)

10 Most Frequently Cited Articles from Stem Cells Journal - Aug 2009

The 10 Most-Frequently Cited Articles
in Stem Cells as of September 1, 2009 -- updated monthly

Most-cited rankings are recalculated at the beginning of the month.
Rankings are based on citations to articles on this journal site from articles in HighWire-hosted journals.

 

1. Bo-Ra Son, Leah A. Marquez-Curtis, Magda Kucia, Marcin Wysoczynski, A. Robert Turner, Janina Ratajczak, Mariusz Z. Ratajczak, Anna Janowska-Wieczorek
  Migration of Bone Marrow and Cord Blood Mesenchymal Stem Cells In Vitro Is Regulated by Stromal-Derived Factor-1-CXCR4 and Hepatocyte Growth Factor-c-met Axes and Involves Matrix Metalloproteinases
  Stem Cells May 01, 2006; 24: 1254-1264.
(In "TISSUE-SPECIFIC STEM CELLS")   [Abstract] [Full Text] [PDF]
  (Cited 33 times)
2. Mauro Krampera, Lorenzo Cosmi, Roberta Angeli, Annalisa Pasini, Francesco Liotta, Angelo Andreini, Veronica Santarlasci, Benedetta Mazzinghi, Giovanni Pizzolo, Fabrizio Vinante, Paola Romagnani, Enrico Maggi, Sergio Romagnani, Francesco Annunziato
  Role for Interferon-{gamma} in the Immunomodulatory Activity of Human Bone Marrow Mesenchymal Stem Cells
  Stem Cells Feb 01, 2006; 24: 386-398.
(In "TRANSLATIONAL AND CLINICAL RESEARCH")   [Abstract] [Full Text] [PDF]
  (Cited 28 times)
3. Susanne Kern, Hermann Eichler, Johannes Stoeve, Harald Klüter, Karen Bieback
  Comparative Analysis of Mesenchymal Stem Cells from Bone Marrow, Umbilical Cord Blood, or Adipose Tissue
  Stem Cells May 01, 2006; 24: 1294-1301.
(In "TISSUE-SPECIFIC STEM CELLS")   [Abstract] [Full Text] [PDF]
  (Cited 27 times)
4. Giselle Chamberlain, James Fox, Brian Ashton, Jim Middleton
  Concise Review: Mesenchymal Stem Cells: Their Phenotype, Differentiation Capacity, Immunological Features, and Potential for Homing
  Stem Cells Nov 01, 2007; 25: 2739-2749.
(In "TISSUE-SPECIFIC STEM CELLS")   [Abstract] [Full Text] [PDF]
  (Cited 25 times)
5. Jakub Tolar, Alma J. Nauta, Mark J. Osborn, Angela Panoskaltsis Mortari, Ron T. McElmurry, Scott Bell, Lily Xia, Ning Zhou, Megan Riddle, Tania M. Schroeder, Jennifer J. Westendorf, R. Scott McIvor, Pancras C.W. Hogendoorn, Karoly Szuhai, LeAnn Oseth, Betsy Hirsch, Stephen R. Yant, Mark A. Kay, Alexandra Peister, Darwin J. Prockop, Willem E. Fibbe, Bruce R. Blazar
  Sarcoma Derived from Cultured Mesenchymal Stem Cells
  Stem Cells Feb 01, 2007; 25: 371-379.
(In "CANCER STEM CELLS")   [Abstract] [Full Text] [PDF]
  (Cited 24 times)

 

6. Masako Miura, Yasuo Miura, Hesed M. Padilla-Nash, Alfredo A. Molinolo, Baojin Fu, Vyomesh Patel, Byoung-Moo Seo, Wataru Sonoyama, Jenny J. Zheng, Carl C. Baker, Wanjun Chen, Thomas Ried, Songtao Shi
  Accumulated Chromosomal Instability in Murine Bone Marrow Mesenchymal Stem Cells Leads to Malignant Transformation
  Stem Cells Apr 01, 2006; 24: 1095-1103.
(In "TISSUE-SPECIFIC STEM CELLS")   [Abstract] [Full Text] [PDF]
  (Cited 24 times)
7. Pär Thored, Andreas Arvidsson, Emanuele Cacci, Henrik Ahlenius, Therése Kallur, Vladimer Darsalia, Christine T. Ekdahl, Zaal Kokaia, Olle Lindvall
  Persistent Production of Neurons from Adult Brain Stem Cells During Recovery after Stroke
  Stem Cells Mar 01, 2006; 24: 739-747.
(In "TISSUE-SPECIFIC STEM CELLS")   [Abstract] [Full Text] [PDF]
  (Cited 24 times)
8. Marek Honczarenko, Yi Le, Marcin Swierkowski, Ionita Ghiran, Aleksandra M. Glodek, Leslie E. Silberstein
  Human Bone Marrow Stromal Cells Express a Distinct Set of Biologically Functional Chemokine Receptors
  Stem Cells Apr 01, 2006; 24: 1030-1041.
(In "TISSUE-SPECIFIC STEM CELLS")   [Abstract] [Full Text] [PDF]
  (Cited 22 times)
9. Donald G. Phinney, Darwin J. Prockop
  Concise Review: Mesenchymal Stem/Multipotent Stromal Cells: The State of Transdifferentiation and Modes of Tissue Repair—Current Views
  Stem Cells Nov 01, 2007; 25: 2896-2902.
(In "TRANSLATIONAL AND CLINICAL RESEARCH: MESENCHYMAL STEM CELLS SERIES")   [Abstract] [Full Text] [PDF]
  (Cited 21 times)
10. Eva Rohde, Christina Malischnik, Daniela Thaler, Theresa Maierhofer, Werner Linkesch, Gerhard Lanzer, Christian Guelly, Dirk Strunk
  Blood Monocytes Mimic Endothelial Progenitor Cells
  Stem Cells Feb 01, 2006; 24: 357-367.
(In "TISSUE-SPECIFIC STEM CELLS")   [Abstract] [Full Text] [PDF]
  (Cited 21 times)

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10 Most Frequently Read Articles from Stem Cells Journal - July 2009

The 10 Most-Frequently Read Articles

in Stem Cells during August 2008 thru July 2009 -- updated monthly

Most-read rankings are recalculated at the beginning of the month and are based on full-text and pdf views.

 

1.     Giselle Chamberlain, James Fox, Brian Ashton, Jim Middleton

Concise Review: Mesenchymal Stem Cells: Their Phenotype, Differentiation Capacity, Immunological Features, and Potential for Homing

Stem Cells Nov 01, 2007; 25: 2739-2749.
(In "TISSUE-SPECIFIC STEM CELLS")

AbstractReferences |  Full Text: HTML, PDF (369K)

(Read 6603 times)

 

2.     Donald G. Phinney, Darwin J. Prockop

Concise Review: Mesenchymal Stem/Multipotent Stromal Cells: The State of Transdifferentiation and Modes of Tissue Repair—Current Views

Stem Cells Nov 01, 2007; 25: 2896-2902.
(In "TRANSLATIONAL AND CLINICAL RESEARCH: MESENCHYMAL STEM CELLS SERIES")  

AbstractReferences |  Full Text: HTML, PDF (127K)

(Read 5989 times)

 

3.     Prashant Mali, Zhaohui Ye, Holly H. Hommond, Xiaobing Yu, Jeff Lin, Guibin Chen, Jizhong Zou, Linzhao Cheng

Improved Efficiency and Pace of Generating Induced Pluripotent Stem Cells from Human Adult and Fetal Fibroblasts

Stem Cells Aug 01, 2008; 26: 1998-2005.
(In "EMBRYONIC STEM CELLS/INDUCED PLURIPOTENT STEM CELLS")

AbstractReferences |  Full Text: HTML, PDF (1644K)

(Read 5898 times)

 

4.     Lindolfo da Silva Meirelles, Arnold I. Caplan, Nance Beyer Nardi

In Search of the In Vivo Identity of Mesenchymal Stem Cells

Stem Cells Sep 01, 2008; 26: 2287-2299.
(In "The Stem Cell Niche")

AbstractReferences |  Full Text: HTML, PDF (465K)

(Read 5015 times)

 

5.     Vinagolu K. Rajasekhar, Martin Begemann

Concise Review: Roles of Polycomb Group Proteins in Development and Disease: A Stem Cell Perspective

Stem Cells Oct 01, 2007; 25: 2498-2510.
(In "STEM CELL GENETICS AND GENOMICS")

AbstractReferences |  Full Text: HTML, PDF (541K)

(Read 4820 times)

 

6.     Peter J. Psaltis, Andrew C.W. Zannettino, Stephen G. Worthley, Stan Gronthos

Concise Review: Mesenchymal Stromal Cells: Potential for Cardiovascular Repair

Stem Cells Sep 01, 2008; 26: 2201-2210.
(In "TRANSLATIONAL AND CLINICAL RESEARCH")

AbstractReferences |  Full Text: HTML, PDF (269K)

(Read 4639 times)

 

7.     Andreas Schäffler, Christa Büchler

Concise Review: Adipose Tissue-Derived Stromal Cells—Basic and Clinical Implications for Novel Cell-Based Therapies

Stem Cells Apr 01, 2007; 25: 818-827.
(In "TISSUE-SPECIFIC STEM CELLS")

AbstractReferences |  Full Text: HTML, PDF (319K)

(Read 4523 times)

 

8.     Stephanie M. Willerth, Tracy E. Faxel, David I. Gottlieb, Shelly E. Sakiyama-Elbert

The Effects of Soluble Growth Factors on Embryonic Stem Cell Differentiation Inside of Fibrin Scaffolds

Stem Cells Sep 01, 2007; 25: 2235-2244.
(In "EMBRYONIC STEM CELLS")

AbstractReferences |  Full Text: HTML, PDF (1176K)

(Read 4475 times)

 

9.     Ornella Parolini, Francesco Alviano, Gian Paolo Bagnara, Grozdana Bilic, Hans-Jörg Bühring, Marco Evangelista, Simone Hennerbichler, Bing Liu, Marta Magatti, Ning Mao, Toshio Miki, Fabio Marongiu, Hideaki Nakajima, Toshio Nikaido, C. Bettina Portmann-Lanz, Venkatachalam Sankar, Maddalena Soncini, Guido Stadler, Daniel Surbek, Tsuneo A. Takahashi, Heinz Redl, Norio Sakuragawa, Susanne Wolbank, Steffen Zeisberger, Andreas Zisch, Stephen C. Strom

Concise Review: Isolation and Characterization of Cells from Human Term Placenta: Outcome of the First International Workshop on Placenta Derived Stem Cells

Stem Cells Feb 01, 2008; 26: 300-311.
(In "TISSUE-SPECIFIC STEM CELLS")

AbstractReferences |  Full Text: HTML, PDF (593K)

(Read 4234 times)

 

10.    Louise C. Laurent, Jing Chen, Igor Ulitsky, Franz-Josef Mueller, Christina Lu, Ron Shamir, Jian-Bing Fan, Jeanne F. Loring

Comprehensive MicroRNA Profiling Reveals a Unique Human Embryonic Stem Cell Signature Dominated by a Single Seed Sequence

Stem Cells Jun 01, 2008; 26: 1506-1516.
(In "EMBRYONIC STEM CELLS")

AbstractReferences |  Full Text: HTML, PDF (1003K)

(Read 4004 times)

 

10 Most Frequently Cited Articles from Stem Cells Journal - July 2009

The 10 Most-Frequently Cited Articles

in Stem Cells as of August 1, 2009 -- updated monthly

Most-cited rankings are recalculated at the beginning of the month.
Rankings are based on citations to articles on this journal site from articles in HighWire-hosted journals.

 

1.     Bo-Ra Son, Leah A. Marquez-Curtis, Magda Kucia, Marcin Wysoczynski, A. Robert Turner, Janina Ratajczak, Mariusz Z. Ratajczak, Anna Janowska-Wieczorek

Migration of Bone Marrow and Cord Blood Mesenchymal Stem Cells In Vitro Is Regulated by Stromal-Derived Factor-1-CXCR4 and Hepatocyte Growth Factor-c-met Axes and Involves Matrix Metalloproteinases

Stem Cells May 01, 2006; 24: 1254-1264.
(In "TISSUE-SPECIFIC STEM CELLS")  

AbstractReferences |  Full Text: HTML, PDF (859K)

(Cited 32 times)

 

2.     Mauro Krampera, Lorenzo Cosmi, Roberta Angeli, Annalisa Pasini, Francesco Liotta, Angelo Andreini, Veronica Santarlasci, Benedetta Mazzinghi, Giovanni Pizzolo, Fabrizio Vinante, Paola Romagnani, Enrico Maggi, Sergio Romagnani, Francesco Annunziato

Role for Interferon- in the Immunomodulatory Activity of Human Bone Marrow Mesenchymal Stem Cells

Stem Cells Feb 01, 2006; 24: 386-398.
(In "TRANSLATIONAL AND CLINICAL RESEARCH")  

AbstractReferences |  Full Text: HTML, PDF (803K)

(Cited 28 times)

 

3.     Susanne Kern, Hermann Eichler, Johannes Stoeve, Harald Klüter, Karen Bieback

Comparative Analysis of Mesenchymal Stem Cells from Bone Marrow, Umbilical Cord Blood, or Adipose Tissue

Stem Cells May 01, 2006; 24: 1294-1301.
(In "TISSUE-SPECIFIC STEM CELLS")

AbstractReferences |  Full Text: HTML, PDF (505K)

(Cited 27 times)

 

4.     Giselle Chamberlain, James Fox, Brian Ashton, Jim Middleton

Concise Review: Mesenchymal Stem Cells: Their Phenotype, Differentiation Capacity, Immunological Features, and Potential for Homing

Stem Cells Nov 01, 2007; 25: 2739-2749.
(In "TISSUE-SPECIFIC STEM CELLS")

AbstractReferences |  Full Text: HTML, PDF (369K)

(Cited 23 times)

 

5.     Jakub Tolar, Alma J. Nauta, Mark J. Osborn, Angela Panoskaltsis Mortari, Ron T. McElmurry, Scott Bell, Lily Xia, Ning Zhou, Megan Riddle, Tania M. Schroeder, Jennifer J. Westendorf, R. Scott McIvor, Pancras C.W. Hogendoorn, Karoly Szuhai, LeAnn Oseth, Betsy Hirsch, Stephen R. Yant, Mark A. Kay, Alexandra Peister, Darwin J. Prockop, Willem E. Fibbe, Bruce R. Blazar

Sarcoma Derived from Cultured Mesenchymal Stem Cells

Stem Cells Feb 01, 2007; 25: 371-379.
(In "CANCER STEM CELLS")  

AbstractReferences |  Full Text: HTML, PDF (861K)

(Cited 23 times)

 

6.     Pär Thored, Andreas Arvidsson, Emanuele Cacci, Henrik Ahlenius, Therése Kallur, Vladimer Darsalia, Christine T. Ekdahl, Zaal Kokaia, Olle Lindvall

Persistent Production of Neurons from Adult Brain Stem Cells During Recovery after Stroke

Stem Cells Mar 01, 2006; 24: 739-747.
(In "TISSUE-SPECIFIC STEM CELLS")  

AbstractReferences |  Full Text: HTML, PDF (1080K)

(Cited 23 times)

 

7.     Masako Miura, Yasuo Miura, Hesed M. Padilla-Nash, Alfredo A. Molinolo, Baojin Fu, Vyomesh Patel, Byoung-Moo Seo, Wataru Sonoyama, Jenny J. Zheng, Carl C. Baker, Wanjun Chen, Thomas Ried, Songtao Shi

Accumulated Chromosomal Instability in Murine Bone Marrow Mesenchymal Stem Cells Leads to Malignant Transformation

Stem Cells Apr 01, 2006; 24: 1095-1103.
(In "TISSUE-SPECIFIC STEM CELLS")  

AbstractReferences |  Full Text: HTML, PDF (976K)

(Cited 22 times)

 

8.     Donald G. Phinney, Darwin J. Prockop

Concise Review: Mesenchymal Stem/Multipotent Stromal Cells: The State of Transdifferentiation and Modes of Tissue Repair—Current Views

Stem Cells Nov 01, 2007; 25: 2896-2902.
(In "TRANSLATIONAL AND CLINICAL RESEARCH: MESENCHYMAL STEM CELLS SERIES")

AbstractReferences |  Full Text: HTML, PDF (127K)

(Cited 20 times)

 

9.     Erica L. Herzog, John Van Arnam, BuQu Hu, Diane S. Krause

Threshold of Lung Injury Required for the Appearance of Marrow-Derived Lung Epithelia

Stem Cells Aug 01, 2006; 24: 1986-1992.
(In "TRANSLATIONAL AND CLINICAL RESEARCH")

AbstractReferences |  Full Text: HTML, PDF (259K)

(Cited 20 times)

 

10.    Marek Honczarenko, Yi Le, Marcin Swierkowski, Ionita Ghiran, Aleksandra M. Glodek, Leslie E. Silberstein

Human Bone Marrow Stromal Cells Express a Distinct Set of Biologically Functional Chemokine Receptors

Stem Cells Apr 01, 2006; 24: 1030-1041.
(In "TISSUE-SPECIFIC STEM CELLS")

AbstractReferences |  Full Text: HTML, PDF (1934K)

(Cited 20 times)

 

The Anti-Inflammatory and Anti-Angiogenic Role of Mesenchymal Stem Cells in Corneal Wound Healing Fo

The Anti-Inflammatory and Anti-Angiogenic Role of Mesenchymal Stem Cells in Corneal Wound Healing Following Chemical Injury

 

Joo Youn Oha, Mee Kum Kima, Mi Sun Shinb, Hyun Ju Leeb, Jung Hwa Kob, Won Ryang Weea, Jin Hak Leea

ABSTRACT

To investigate the anti-inflammatory and anti-angiogenic effects of mesenchymal stem cells (MSC) in the chemically burned corneas, we mechanically removed the corneal epithelium of rats after 100% alcohol instillation. The rats were then randomized into four groups: fresh media, conditioned media derived from the MSC culture (MSC-CM), MSC applied topically to the damaged corneas for 2 hours immediately after the injury or MSC-CM applied either once or 3 times per day for 3 consecutive days. Corneal surface was evaluated every week. After 3 weeks, the corneas were stained with the hematoxylin-eosin, and the expression of interleukin (IL)-2, interferon (IFN)-, IL-6, IL-10, transforming growth factor (TGF)-β1, thrombospondin-1 (TSP-1), matrix metalloproteinase-2 (MMP-2), and vascular endothelial growth factor (VEGF) were analyzed. CD4+ cells were assessed in the corneas. We found that both MSC and three-time applied MSC-CM (1) reduced corneal inflammation and neovascularization, (2) decreased IL-2 and IFN-, although increased IL-10 and TGF-β1 as well as IL-6, (3) reduced the infiltration of CD4+ cells, and (4) upregulated the expression of TSP-1, although downregulated that of MMP-2. Interestingly, whereas three-time application of MSC-CM was partially effective, transplantation of MSC achieved a better outcome in suppressing corneal inflammation. The results of this study suggest that the anti-inflammatory and anti-angiogenic action of MSC in the chemically burned corneas might be mediated in part through paracrine pathways involving soluble factors such as IL-10, TGF-β1, IL-6 and TSP-1.

Disclosure of potential conflicts of interest is found at the end of this article.

AbstractReferences |  Full Text: HTML, PDF (1586K)

Transplantation of Embryonic Stem Cells Improves Nerve Repair and Functional Recovery After Severe S

Transplantation of Embryonic Stem Cells Improves Nerve Repair and Functional Recovery After Severe Sciatic Nerve Axotomy in Rats

Lin Cuia,c, Jun Jianga,c, Ling Weia,d, Xin Zhoub, Jamie L. Frasera, B. Joy Sniderd, Shan Ping Yua,b

ABSTRACT

Extensive research has focused on transplantation of pluripotent stem cells for the treatment of central nervous system disorders, the therapeutic potential of stem cell therapy for injured peripheral nerves is largely unknown. We used a rat sciatic nerve transection model to test the ability of implanted embryonic stem (ES) cell-derived neural progenitor cells (ES-NPCs) in promoting repair of a severely injured peripheral nerve. Mouse ES cells were neurally induced in vitro; enhanced expression and/or secretion of growth factors were detected in differentiating ES cells. One hour after removal of a 1-cm segment of the left sciatic nerve, ES-NPCs were implanted into the gap between the nerve stumps with the surrounding epineurium as a natural conduit. The transplantation resulted in substantial axonal regrowth and nerve repair, which were not seen in culture medium controls. One to 3 months after axotomy, co-immunostaining with the mouse neural cell membrane specific antibody M2/M6 and the Schwann cell marker S100 suggested that transplanted ES-NPCs had survived and differentiated into myelinating cells. Regenerated axons were myelinated and showed a uniform connection between proximal and distal stumps. Nerve stumps had near normal diameter with longitudinally oriented, densely packed Schwann cell-like phenotype. Fluoro-Gold retrogradely labeled neurons were found in the spinal cord (T12–13) and DRG (L4-L6), suggesting reconnection of axons across the transection. Electrophysiological recordings showed functional activity recovered across the injury gap. These data suggest that transplanted neurally induced ES cells differentiate into myelin-forming cells and provide a potential therapy for severely injured peripheral nerves.

 

AbstractReferences |  Full Text: HTML, PDF (3567K)

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Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate Autoimmune Enteropathy

Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate Autoimmune Enteropathy Independently of Regulatory T Cells

Biju Parekkadana,b, Arno W. Tillesa, Martin L. Yarmusha,b

ABSTRACT

Cell-based tolerogenic therapy is a relatively new approach for the treatment of autoimmune diseases. Mesenchymal stem cells (MSCs) have been shown to be potent immunomodulatory agents in a number of experimental and clinical scenarios; however, their use in various autoimmune diseases is undefined. Herein, we report the efficacy of MSC transplantation in a multiorgan autoimmunity model. Mice with defective peripheral tolerance caused by a deficiency in regulatory T cells were used as a testbed for therapy. After screening multiple target tissues of autoimmune attack, we observed an MSC-specific improvement in the histopathology of the distal ileum of treated mice. We then showed that MSCs can reduce mesenteric lymph node (MLN) cellularity in autoimmune mice during active disease and decrease activated T-cell populations in the MLN. Trafficking studies using enhanced green fluorescent protein (eGFP)-reporter MSCs revealed no appreciable engraftment in the intestine, but it did reveal the presence of eGFP+ cells organized in clusters within the MLN, as well as ancillary nodes. Semiquantitative analysis showed no difference in the number of clusters; however, eGFP+ cells in MLNs compared with ancillary nodes had distinct fibroblastoid morphology and formed a network with neighboring eGFP+ cells. Finally, we show evidence that transplantation of MSCs caused global immunosuppression, as measured by increased CD4+ CD8+ thymocyte production and serum interleukin-10 and decreased serum interferon-gamma. These data implicate the intestine as a new site of MSC tolerance induction and should motivate additional studies evaluating the use of MSCs as a treatment for autoimmune enteropathies.

 

AbstractReferences |  Full Text: HTML, PDF (927K)

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