Enhancing In Vivo Survival of Adipose-Derived Stromal Cells Through Bcl-2 Overexpression Using a Minicircle Vector
From Stem Cells Translational Medicine
Survival of therapeutically relevant multipotent stem cells, such as human adipose-derived stromal cells (hASCs), is currently a significant hurdle to stem cell therapy. This barrier is primarily caused by environmental stresses on transplanted cells by the often refractory cellular environment on the transplant site (Allen et al, Bauer et al and Broughton et al). This often leads to apoptosis (Zhang et al) and poor engraftment (Wu et al), and therefore it has been postulated that modulating the apoptosis pathway may enhance the therapeutic worth of stem cells. In a study published in Stem Cells TM, the group of Michael T. Longaker at the Stanford University School of Medicine, USA have studied the overexpression of the Bcl-2 gene, known to imbue survival advantages (Domen and Domen and Weissman), in hASCs and demonstrated an increased level of tissue regeneration which can be translated to a clinical setting (Hyun et al).