This article reports an improvement in CRISPR/Cas9‐mediated gene editing outcomes in human hematopoietic stem cells. The results of this study show that cell cycle‐dependent control of nuclease activity and DNA repair pathways can influence gene editing outcomes to favor the precise DNA modification over faulty repair events in human hematopoietic stem cells. This work provides important proof‐of‐principle findings and can improve gene editing outcomes for the treatment of congenital diseases of the blood system.
Based on transcriptomic data on single‐cell derived clones, this study identified CD56 as a potential predictive marker able to select human bone marrow mesenchymal stromal cells (BMSC) subpopulations with higher and more predictable chondrogenic capacity, although still highly affected by donor‐to‐donor variability. Even if selection of this single marker (i.e., CD56) for distinguishing BMSC chondrogenic subpopulations may drastically increase the clinical relevance of this approach, the intrinsic complexity and plasticity of the BMSC system challenges this conclusion. This awareness should alert the field about the difficulty of identifying univocal pre‐selection criteria following the current approaches, while possibly opening the path to innovative computational methods eventually able to process multiple variables of the BMSC system.
Researchers discover a novel lncRNA linked to the osteogenesis of bone marrow MSCs and also describe the crucial interaction of this lncRNA with hnRNPK